American 3i diSPIM Dual-view Inverted Sciective Plane Illumination

diSPIM Dual-view Inverted Sciective Plane Illumination

BRIEF

Dual-view Inverted Selective Plane Illumination (diSPIM) is a light sheet method offering low phototoxicity in 3D imaging making it possible to image living small organisms over extended periods of time. It is a flexible light sheet system with an open specimen geometry not requiring special slides or capillaries. diSPIM images a specimen using two perpendicular light sheets from two equivalent optical paths and objectives which are each used sequentially for both illumination and imaging. The resulting 3D datasets have isotropic resolution in X, Y and Z with only two views, resulting in fast image capture with low light dose for extended specimen viability.

Two matching water dipping objectives can have an NA of up to 0.8 with a working distance allowing efficient orthogonal mounting. Each objective is used for excitation and emission collection via identical high-speed high-resolution sCMOS cameras. A third objective is located below the specimen chamber for locating the sample. Photomanipulation can be added via the third objective, such as the Ablate photoablation system.

diSPIM is available as a component for theMarianassystem, an addition to an existing research inverted microscope, or as a stand-alone system. When added to an inverted microscope the transmitted light path is replaced by the diSPIM assembly and the microscope’s objective is used for locating the sample.

SlideBook™ 6 software controls the diSPIM microscope, laser launch and sCMOS cameras allowing for intricate high-speed synchronization of all hardware components along with the ability to deconvolve raw data and view 3D renderings of the sample

CORE COMPONENTS

	Research Inverted Microscope		LED Light Source
	Fully motorized and capable of autofocus and all advanced techniques, with a full suite of world class objectives and filters		High power 6-channel LED light source
	diSPIM Microscope		LaserStack + FiberSwitcher
	Two galvo-scanned perpendicular light paths with matching filters and objectives		Modular solid state laser launch with <1ms changing between SDC, TIRF and Photomanipulation
	XYZ Stage		Computer
	Fully motorized specimen platform with options for linear encoder sensitivity and piezo z focus		Dell professional grade workstations offer best-in-class speed and large dataset handling
	Cameras		Digital Microscopy Software
	Dual high speed high resolution sCMOS cameras and another camera for finding the sample via the epi-path		SlideBook offers advanced acquisition via control of hundreds of devices, in addition to advanced analytical techniques and 3D rendering.

PHOTOMANIPULATION