VIVO 2-PhotonTM World’s most flexible multiphoton microscopy platform for neuroscience studies
BRIEF
The VIVO 2-Photon platform incorporates advanced optics, lasers, scanners, PMTs, computers and proprietary electronics to achieve unparalleled speed, precision and flexibility in multiphoton image acquisition. Innovative SlideBook software works seamlessly within VIVO 2-Photon for powerful and intuitive image acquisition and analysis
CORE COMPONENTS
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Microscope Body and Objective |
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Digital Microscopy Software |
Modified Zeiss Axio Examiner Z1 frame body and 20x/1.0 long working distance water immersion objective.
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Advanced acquisition and analysis software for biomedical research that is easy-to-use and powerful.
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2P Vector Scanner |
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Piezo Z Focus |
High-speed 6fps @ 512x512 and 3,000lps with bi-directional scanning for maximum acquisition speed. Patterns include frame, line, curve, multi-region, multi-point, and precisely timed excursions for photostimulation and laser ablation or scission.
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400 micron total travel with 1.25nm step size and 5nm repeatability (microscope focus travel13mm with 50nm step size.) |
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Tunable Ti:Sapphire Lasers |
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mBeam |
Pockels cell control of up to two lasers with 200kHz response. |
Beam expansion and modification for shaping multiphoton laser sources and coupling efficiently to various objective back aperture diameters |
DETECTORS
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Nosepiece |
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Remote PMT Array |
Dual 14-bit GaAsP PMTs collect reflected light close to the sample for maximal sensitivity. |
High sensitivity Peltier-cooled GaAsP PMTs allow for additional acquisition channels. |
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IR Transmission Option |
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Sub-stage Detectors |
Bialkali PMT in unit mounted at transmitted illumination port (shared with transmitted LED by a beamsplitter). |
Up to 2 GaAsP PMTs located below the stage to collect maximal transmitted light via 1.2NA water immersion condenser. |
STAGES
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Platform Stage |
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Luigs & Neumann |
Stable, fixed, large working area stage compatible with mounting multiple manipulators and large trays for tissue and animal presentation |
Highly flexible stages, specimen platforms and manipulators for complex specimen micromanipulation. |
ADVANCED TECHNOLOGIES
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Resonant Scanning |
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Temporal Focusing for 2P Phasor |
High speed resonant scanning of up to 30fps at 2048 x2048 inframe and line patterns. |
Disperses laser illumination above and below the plane of focus dramatically reducing unwanted fluorescence excitation and ensuring Phasor illumination of larger regions or cells deep in scattering tissue are still axially confined to the desired target.
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1P and 2P Phasor |
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Digital holography pattern photomanipulation in X,Y and Z without intensity loss |
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2pFLIM |
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Widefield Imaging |
Using either integrated GaAsP detectors or hybrid PMT/APDs, photon counting hardware, and the SlideBook™ 2pFLI M module |
Camera options include EMCCDs and high-resolution sCMOS cameras for fast switching between widefield and multiphoton imaging. |