Advanced fluorescence microscopy platform (Customizable)

PLATFORMS

VIVO 2-Photon

MARIANAS

VIVO

EVEREST

Fixed-stage upright microscope

Platform designed for highspeed

multiphoton imaging of live animals and tissue

Inverted microscope platform

designed for both live and fixed cell applications

Fixed-stage upright microscope platform ideal for intravital imaging and electrophysiology

Upright microscope platform

optimized for convenient fixed

specimen work

METHODS

Confocal Imaging

Fluorescence Lifetime Imaging

Photomanipulation/FRAP

Photoablation

Ratiometric Imaging

FRET

TIRF

7D Imaging

Fluorescence Correlation

Spectroscopy

Optogenetics

High Content Screening

Photoactivation

Photoswitching

Photostimulation

SOFTWARE

SlideBook software addresses the entire experimental process in biomedical imaging. From hardware device control in acquisition to mid-experiment data analysis to offline image processing and analysis, SlideBook allows users to focus on scientific investigation rather than instrument functionality.

SlideBook 5 comes standard with a large number of acquisition drivers to control over 100 devices from dozens of companies. Data can be acquired in 3D, over time, multiple color channels and as fluorescence lifetimes. Also standard are a wide variety of analytical tools for image processing, mathematical operations and a full suite of statistics functions.

SlideBook 5.5 adds numerous enhancements:

• LaserStream multichannel streaming capture

• Extended interoperability with MATLAB ®3D Deconvolution

• 6D Multicapture allows variation in capture parameters at eachX/Y position

• Improved saving of multi-gigabyte datasets

Modules available for advanced applications:

AutoQuant Blind Deconvolution

Ratio Imaging

FRET

Rapid 4D Streaming

Synchronization of TTL Devices

CellNet™Pattern Recognition

Photomanipulation/FRAP Imaging

Stereology

Super-Resolution Particle Tracking

Spinning Disk Confocal

CSU-X1

• Best combination of speed and

confocality for fast 3D imaging

• Optimized for high NA objectives

• Minimal photodamage for living cells

CSU-W1

• Larger field of view accommodates

sCMOS cameras

• Additional disk available optimized

for lower NA objectives

• Integrated disk movement for single

camera confocal and widefield imaging

CSU-X1            CSU-W1

Pancreatic Islet Cell

Pinhole Diameter

50µm disk

25µmdisk and 50µmdisk

Number of Disks

One

One or two with motorized switching

Disk Bypass

With 3i Bypass™

Standard

Acquisition Speed

2000 FPS

200 FPS

Field of View

10mm x7mm

17mm x16mm

Near IR Excitation

Up to 640nm

Up to 785nm

LaserStack

Modular Laser Light Source

• Solid state laser delivery with

microsecond timing

• Modular form factor allows for

expansion to additional wavelengths

• 405, 445, 473, 488, 514, 532, 561, 594, 640 and 660nm lines available

FiberSwitcher

• Sub-millisecond switch times

between SDC, TIRF, FRAP andFLIM

• 4 fiber-coupled outputs

GFP mouse embryo, 100μm imaging

depth, 40x objective

without SAC(left), with SAC(right)

mSwitcher

Optical Path Selector

• Millisecond switching between up to 3 devices and the microscope

• 1x or 1.2x magnification(s)

mSAC

Spherical Aberration Correction

• Corrects for sample and preparation-induced spherical aberration

• Improves geometric fidelity, resolution at depth and signal-to-noise

• High speed on the fly correction as 3D data is collected

Vector

Modular High Speed X/Y Scanner

• Accepts lasers from UV to IR

• FRAP, photoactivation, photoablation, uncaging

• Multiphoton beam scanning

GFP in trout cells, bleached with a 488nm laser

Phasor

Digital Holography System

• Simultaneous stimulation of

non-adjacent regions in x, y and z

• Optogenetic and

channelrhodopsin studies

preBotC network with neurons selected for simultaneous  photo-

stimulation via Phasor to elicit a network burst.

Ablate!

Laser Ablation System

• Attenuatable 532nm pulse laser

• Localized damage tointracellular

structures & targeted wound control

• 70 µJ peak energy, 100Hz peak rate

Bottom trace represents the laser

pulse and the top trace is a recording

of the hypoglossal nerve (the output

of the preBotC network). Arrows note

endogenous bursts; evoked bursts

indicated by the laser pulse.

Image and data courtesy Dr. Jason Worrell,UCLA

Bifurcator

Optical Splitter and Combiner

• Creates a collimated beam

space for optical accessories

• Ideal for emission filter

wheels

• 1x or 1.2x magnification(s)

Optimizer

Perfect Image Relay

• Optimal positioning

of emission filter

wheels in a collimated

infinity space

• 1x or 1.2x magnification

mFilter

Emission Filter Switcher

• Millisecond switching

among four positions

• User-exchangeable

25mm filters

• Perfect image relay

C．elegans FRET

TIRF

Total Internal Reflection Fluorescence

• Manual or motorized TIRF illuminators and a selection of high NA (>1.40) objectives

• Millisecond wavelength selection via LaserStack

FLIM

Fluorescence Lifetime Imaging

• Widefield frequency-domain detection

• High frequency modulated laser light source and phase-shifted intensifiedCCD detector

• Combinable with widefield fluorescence, TIRF, FRAP and SDC

• Sub-second acquisition

FRET

Förster energy resonance transfer

• Supports dual camera capture and image splitting devices for real time analysis

• Graphs displayed and updated during capture

• Fluorescence anisotropy measurement available